Figure 5. Duration of induced thermotolerance persists until day 8 of adulthood. To determine the duration of induced thermotolerance, worms were exposed to a mild 35°C heat stress for 2 hours on day 1 of adulthood. Resistance to a subsequent 37°C heat stress was then tested at increasing ages (Day 2, Day 4, Day 6, Day 8 and Day 10). Worms treated with a mild heat stress maintain increased resistance to heat stress for at least 8 days. Error bars indicate SEM. *p<0.05, **p<0.01, ***p<0.001.