Figure 8.Insulin downregulated PHLPP-1 protein level through β-TrCP-mediated UPS(A) Representative immunoblots of ubiquitination and PHLPP-1 level with insulin and MG132 treatment. Cardiomyocytes were pre-treated with MG132, 30 min prior to H/R. (B) Quantification of PHLPP-1 level immunoblots results (*P < 0.05 vs. Con; #P < 0.05 vs. H/R; †P < 0.05 vs. H/R+Ins. Values are means ± S.E., n = 5 per group). (C) E3 ligase β-TrCP knockdown terminated degradation of PHLPP-1 with insulin treatment.
