Research Paper Volume 8, Issue 5 pp 873—887
Mutual inhibition of insulin signaling and PHLPP-1 determines cardioprotective efficiency of Akt in aged heart
Figure 6. Exercise training reversed response to insulin of aged hearts. Aged rats were experienced exercise training for 10 weeks. (A) Intraperitoneal glucose tolerance test (IPGTT) and (B) insulin sensitivity test (IST) determined in rats of the indicated group (*P < 0.05 vs. untrained aged group). (C) Trained or untrained aged hearts were subjected to in vivo ischemia and reperfusion. Immunoblots showing PHLPP-1 and Akt phosphorylation. (D-E) Quantification of PHLPP-1 and Akt phosphorylation immunoblots results (*P < 0.05 vs. untrained aged+ I/R+Ins; #P < 0.05 vs. trained aged+I/R). (F) Cardiomyocyte apoptosis determined by TUNEL (*P < 0.05 vs. untrained aged+ I/R; #P < 0.05 vs. trained aged+I/R, Values are means ± S.E., n = 5 per group).