Research Paper Volume 8, Issue 4 pp 712—728

Figure 4. RNA-sequencing reveals specific changes in hindlimb muscles with age. RNA-Seq profiling was performed comparing gastrocnemius and triceps brachii from adult, early sarcopenic or sarcopenic rats. (A) Venn diagrams of genes significantly regulated between the 3 age groups in gastrocnemius and triceps brachii for the two muscles separately. Genes were considered significantly regulated when the absolute fold change was > 2 and the adjusted p-value < 0.05. (B) Venn diagram comparing the overlap of gene regulation with age between gastrocnemius and triceps brachii. Genes were considered significantly regulated when the absolute fold change was > 2 and the adjusted p-value < 0.05. (C) Heat map for neuromuscular function related genes isolated from Gene Ontology enrichment analysis. (D) Validation of key genes expression by qPCR, using HPRT expression for normalization. For all graphs n=9-10 per group. * p<0.05 ** p<0.01 *** p<0.001 with a one-way ANOVA test.