Research Paper Volume 8, Issue 5 pp 1083—1098
Lack of collagen VI promotes neurodegeneration by impairing autophagy and inducing apoptosis during aging
Figure 1. Collagen VI immunolabeling in primary neural cell cultures. (A) Confocal microscopy analysis of immune-fluorescence for collagen VI in primary neural cell cultures. Collagen VI (red) is detected in wild-type, but not in Col6a1−/− cultures. Nuclei were stained with Hoechst (blue). Scale bar, 100 μm. (B) Immunofluorescence for collagen VI (red) and βIII-tubulin (green). In wild-type cultures, collagen VI labelling is detected in both neuronal (βIII-tubulin-positive, arrows) and glial (βIII-tubulin-negative, arrowhead) cells. Nuclei were stained with Hoechst (blue). Scale bar, 100 μm. ColVI, collagen VI; WT, wild-type.