Research Paper Volume 8, Issue 1 pp 50—57

Figure 4. Early exposure to glucose sensitizes nematodes to UPR^mt activation. (a-d) Early exposure (L4) to glucose at different concentrations (1, 2 and 4%) had no effect on hsp-6 expression during adulthood compared to control NGM conditions. RNAi against cco-1 was able to induce hsp-6 expression in animals treated at all glucose concentrations until day 5 of adulthood, and only at 2% and 4% at day 9 (mean ± SD., *P<0.05, **P<0.01 and ****P<0.0001 compared to control). (e) The lifespan on N2 worms treated with cco-1(RNAi) during adulthood was similar to N2 control worms. The lifespans of N2 worms grown on (f) 2% or g. 4% GE plates was reduced when treated with adult-stage specific cco-1(RNAi) compared to worms treated with EV (P<0.01 for 2% GE + cco-1(RNAi) and P<0.0001 for 4% GE + cco-1(RNAi) versus controls.)