Research Paper Volume 7, Issue 11 pp 903—910

Figure 3. Detection of age-dependent changes in circRNA. (A) Schematic of the design of divergent primers used for the specific detection of circRNAs. (B) Example of amplification plots of circRNAs in total RNA isolated monkey muscle tissues. (C) RT-qPCR products visualized by electrophoresis in ethidium bromide-stained agarose gels. (D) Representative examples of qPCR products purified and sequenced to confirm circRNA junction sequences; #, circular intronic (ci)RNAs. (E) For a subset of circRNAs that could be detected following the criteria outlined in the text (number of reads, pattern of dissociation curves, and single amplification products) the relative abundance in Y, MA, and O groups was quantified by RT-qPCR analysis. A circRNA that did not change with muscle age (mmu_circ_004374) was included. *, p<0.05.