Figure 1.Circular RNA identification in skeletal muscle(A) Workflow of the preparation and analysis of circRNAs. After homogenization of skeletal muscle samples, total RNA was extracted and digested with RNase R to degrade linear RNA. Following sequencing (RNA-Seq), sequences were processed, aligned to linear transcripts, assembled into collections of known and novel circRNAs, and annotated. (B) Representative UCSC genome browser views of mmu_circ_017332 (top), mmu_circ_014269 (middle), and the intronic circ-RNA mmu_circ_006990 (bottom), including fine-scale alternate splicing patterns that generate additional circRNA isoforms.
