Deletion of caspase-8 in mouse myeloid cells blocks microglia pro-inflammatory activation and confers protection in MPTP neurodegeneration model

Edel Kavanagh; Miguel Angel Burguillos; Alejandro Carrillo-Jimenez; María José Oliva-Martin; Martiniano Santiago; Johanna Rodhe; Bertrand Joseph; Jose Luis Venero

doi:doi:10.18632/aging.100805

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Research Paper Volume 7, Issue 9 pp 673—689

Deletion of caspase-8 in mouse myeloid cells blocks microglia pro-inflammatory activation and confers protection in MPTP neurodegeneration model

Figure 7. Caspase-8 deficiency protects the nigro-striatal dopaminergic system against the MPTP-induced neurodegenerative changes. Illustration of tyrosine-hydroxylase (TH) immunoreactivity in substantia nigra (a) and striatum (c) in response to either saline or MPTP in Casp8^fl/fl and Cre^LysMCasp8^fl/fl mice. Stereological quantification of TH-labeled neurons of each condition is shown in (b). There was less loss of nigra dopaminergic neurons in response to MPTP in Cre^LysMCasp8^fl/fl mice, this effect being close to statistical significance. Analysis of integrity of striatal dopaminergic nerve endings (d) demonstrated significantly less MPTP-induced loss of TH immunoreactivity in Cre^LysMCasp8^fl/fl mice as compared with Casp8^fl/fl mice (p <0.05). Scale bar: a: 400 μm; c: 500 μm.