Functional genomic analysis reveals overlapping and distinct features of chronologically long-lived yeast populations

Margaret B. Wierman; Mirela Matecic; Veena Valsakumar; Mingguang Li; Daniel L. Smith; Stefan Bekiranov; Jeffrey S. Smith

doi:doi:10.18632/aging.100729

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Research Paper Volume 7, Issue 3 pp 177—194

Functional genomic analysis reveals overlapping and distinct features of chronologically long-lived yeast populations

Figure 3. Overlapping effects of isonicotinamide (INAM) and expired CR media concentrate on CLS and gene expression. (A) Isonicotinamide (25 mM), extends CLS of WT yeast through a SIR2 independent mechanism. (B) Venn diagrams showing the number of genes found to be commonly up‐ or downregulated among multiple longlived conditions – CR conc., ade4∆, INAM, and CR– when compared to WT NR day 8 stationary cell populations. (C) Heat map of gene expression fold‐changes (log₂) induced by INAM or the CR concentrate relative to WT. (D) Scatter plot of gene expression fold‐changes (log₂) between day 8 cultures supplemented with the CR concentrate vs. INAM.