Research Paper Volume 7, Issue 1 pp 53—69

Figure 5. Effect of mifepristone on feeding and progeny production. (A) Feeding assay. The indicated crosses were conducted and virgin females were collected over 24 hours and then mated to w[1118] males at a ratio of 1:1 for 4 days. Mated females were then separated from the males and cultured as adults in the presence and absence of 160ug/ml drug for 20 days. Feeding was estimated using dye uptake assay, using 6 biological replicates per sample, and 5 flies for each replicate. Plus-drug was compared to minus-drug using unpaired, two-sided t-tests, and statistically significant differences (p < 0.05) are indicated with asterisk. (B) Progeny production. The strain p53B[6] was crossed to rtTA(3)E2 and the female progeny were collected as virgins over 24 hours, and then mated to w[1118] males at a ratio of 1:1 for 4 days. Mated females were then separated from the males and cultured as adults in the presence and absence of 160ug/ml drug. 5 replicate vials of 20 females per vial. Number offspring per female is plotted as average +/− SD. Plus-drug compared to minus-drug using unpaired, two-sided t-tests, statistically significant difference (p < 0.05) indicated with asterisk.