Research Paper Volume 6, Issue 12 pp 1076—1093
Embryonic expression of shuttle craft, a Drosophila gene involved in neuron development, is associated with adult lifespan
Figure 1. stc gene map and PCR analysis of stc structure in revertant lines. (A) stc gene map. Gray arrows: exons; black sections:introns; gray sections: untranslataed regions; pink sections: background sequences; red arrow: P{SUPor-P}stcKG01230 insertion site; black arrows: primers used for PCR; gray arrows: primers used for Real Time RT-qPCR. (B) PCR with primers pstc1 and pstc2 (expected product size 555 bp); (C) PCR with primers pstc0 and pstc02 (expected product size 2508 bp); (D) PCR with primers pstc3 and pstc4 (expected product size 2562 bp). 1: control line; 2: rev1, 3: rev3, 4: rev4; 5: rev5.