Research Paper Volume 6, Issue 12 pp 1049—1063

Figure 4. Progerin expression decreases MIAMI cell proliferation. (A) Quantification of Ki67 immunofluorescent staining. At least 10 random fields per cell line were selected and cells were scored as positive or negative for Ki67 expression. Progerin expression significantly decreases Ki67 expression when compared to lamin A MIAMI cells in the same passage. (B) Immunofluorescent images of transgene and Ki67 expression, a marker of proliferation, in lamin A and progerin MIAMI cells. (C) Growth curve of untransduced MIAMI cells, vector control, lamin A, and progerin MIAMI cells. Progerin expression significantly decreases cell number at days 3, and 5 when compared to control cells. There were no significant differences between the control cell lines. Values are mean ± standard deviation (n≥3). *p<0.05, calculated by Student's t-test.