Research Paper Volume 6, Issue 12 pp 1049—1063

Figure 2. MIAMI cells can express endogenous progerin. (A) Endogenous progerin mRNA levels measured by qPCR in MIAMI cells collected from non-HGPS male donors of various ages (7-65 years). Progerin-transduced MIAMI cells collected from a 20-year-old donor (+) were used as a positive control. MIAMI cells collected from a 20 year old (−) were used as a negative control. (B) Using primer pairs to amplify both progerin and lamin A, qPCR results were run on an agarose gel to visualize amplification product length. Miami cells collected from a 65-year-old donor and progerin transduced MIAMI cells express both lamin A and progerin products. Values in Panel A are mean ± standard deviation (n≥3). *p<0.05, calculated by Student's t-test. P-values indicate significant difference between negative control, unless otherwise noted.