Research Paper Volume 6, Issue 12 pp 1094—1108

Figure 7. y- and a-iPSCs present differences in basal MMP and following H₂O₂ exposure. (A) JC-1 staining images before and after 5, 10 and 15 minutes after H₂O₂ exposure in y-iPSCs. (B) JC-1 staining images before and after 1 and 5 minutes after H₂O₂ exposure in a-iPSCs. Regions of high mitochondrial polarization are revealed by red fluorescence due to J-aggregate formation of the concentrated dye, whereas depolarized regions are indicated by green fluorescence of JC-1 monomers. Pictures are representative images chosen among 3 independent experiments performed in live imaging condition where CO₂ (5%) and Temperature (37°C) were controlled using a top stage incubator. Magnified views of the boxed area are displayed as Insets in A and B. Scale bar: 75 μm. (C) Time course of y- and a-iPSCs before and during H₂O₂ treatment showing the fluorescence intensity ratio (expressed as %) for JC-1 staining (Red/Green fluorescence intensity ratio).