Research Paper Volume 6, Issue 12 pp 1019—1032

Figure 4. Ablation of GHS-R activates thermogenic signaling cascade and improves mitochondrial dynamics of BAT in aged mice. The representative data presented here were from 22 month-old WT and Ghsr^−/− mice. (A) Representative Western blots of key thermogenic regulators in BAT of aged WT and Ghsr^−/− mice. (B) Ex vivo lipolysis of BAT of aged WT and Ghsr^−/− mice, treated with or without 10 μM CL316243. (C) Representative Western blots show phosphorylated AMPK α (p-AMPK-α), total AMPK (t-AMPK-α) and UCP1 protein expression in BAT of old WT and Ghsr^−/− mice after 4 hours of cold exposure. (D) Expression of mitochondrial fission genes in BAT of aged WT and Ghsr^−/− mice. (E) Expression of mitochondrial fusion genes in BAT of aged WT and Ghsr^−/− mice. (F) Expression of mitochondrial complex biogenesis markers in BAT of aged WT and Ghsr^−/− mice. N = 6. *p<0.05, **p<0.001, WT vs. Ghsr^−/−; #p<0.05, basal vs. CL316243 treatment.