Research Paper Volume 6, Issue 11 pp 957—969

Figure 2. Effect of increased metabolic activity on gene expression profile in CD4+ T lymphocytes. RNA samples from 23 donors (age range 29-82) (Table S1C) were obtained from freshly purified CD4⁺ T cells (maintained at 4°C) or from cells incubated at 37°C for 4h. The 46 samples were converted to biotin labeled cRNA and hybridized to Illumina HumanRef-8 Expression BeadChip. (A) Normalized data were analyzed by PAGE and the average Z-score for individual pathways compared between 4°C samples and those incubated at 37°C to obtain a Z-score difference x-axis; Z-score (37°C-4°C ). Rows denote individual pathways. Most prominently up- and down-regulated pathways at 37°C are indicated in red. (B) and (C) Statistically significant genes within the NF-κB induce (up-regulated) and BCR (down-regulated) pathways.