Research Paper Volume 6, Issue 11 pp 931—942

Figure 3. Primary Nfkb1^−/− MEFs exhibit premature cellular senescence. (A) Growth curves of primary Nfkb1^−/− and Nfkb1^+/+ MEFs (n=3 separate MEF isolates per group, harvested at different times) starting at PD5 after harvest (set as time 0). (B) SA-β-gal staining in Nfkb1^−/− and Nfkb1^+/+ MEFs at the indicated passage. n=3 per group. Representative image shown (right). (C) Immunoblot using anti-p16 and anti-p21 antibody in primary Nfkb1^−/− and Nfkb1^+/+ MEFs at passage 4, 5 and 6. (D) Quantification of percent BrdU-positive cells at the indicated passage. n=3 isolates per group, harvested at different times. Data represent mean +/− SEM. *p<0.05; **p<0.01.