Research Paper Volume 6, Issue 11 pp 921—930

Figure 1. TAp73 overexpression induces nucleotide monophosphates. TAp73β overxpression in SaOs-2 Tet-On cell lines results in a highly significant enrichment in all nucleotide monophosphates, consistent with an increased metabolic demand to sustain cell growth. (a) adenosine 5′-monophosphate (AMP), (b) guanosine 5′-monophosphate (5′-GMP), (c) cytidine 5′-monophosphate (5′-CMP), (d) thymidine 5′-monophosphate, (e) uridine monophosphate (5′ or 3′-UMP). Analysis was performed on thirty million cells per samples, 10 samples were analyzed for each time point (n=10). All the samples were extracted using standard metabolic solvent extraction methods and analyzed through GC/MS and LS/MS as previously described [44]. Box indicates upper/lower quartile, bars max/min of distribution. ** p<0.05; * 0.05<p<0.10.