Research Paper Volume 6, Issue 10 pp 835—851

Figure 5. PHA-4 controls a large number of genes by feed-forward loops using miRNAs. (A) A representation of transcription factor-miRNA feed forward loop involving PHA-4 that controls life span during DR. (B) A complex regulatory network involving PHA-4 and its target mRNAs that are also regulated by PHA-4-controlled miRNAs. The network was generated using Cytoscape v3.1.1. Only a subset of genes targeted by PHA-4-regulated miRNA [as determined by mirTarBase were used to generate a representative decongested network. The boxes corresponding to the miRNA or mRNA/genes are colored based on their fold induction in eat-2(ad1116) as compared to WT. Edges are colored based on the number of miRNAs regulating a particular gene. Dark red are for genes regulated by 20 or more miRNAs while those regulated by less than 10 miRNAs are in black. MiRNAs that have no target listed in mirTarBase are in red. (C) The genes targeted by PHA-4 transcriptionally as well as post-transcriptionally are highly connected by protein-protein interactions, as determined by STRING software.