Research Paper Volume 6, Issue 10 pp 835—851
PHA-4/FOXA-regulated microRNA feed forward loops during Caenorhabditis elegans dietary restriction
Figure 4. The miRNAs and mRNAs upregulated during DR are potential direct targets of PHA-4. (A) QRT-PCR analysis shows the expression levels of the miRNAs in eat-2(ad1116);rrf-3(pk1246) compared to rrf-3(pk1246), when grown on control or pha-4 RNAi. (B) QRT-PCR analysis showing the expression levels of predicted PHA-4 direct target genes in eat-2(ad1116);rrf-3(pk1246) compared to rrf-3(pk1246), grown either on control and pha-4 RNAi. The error bars represent Standard Deviation between independent biological samples. ‘ns’ represents p ≥ 0.05 by t-test.