Research Paper Volume 6, Issue 10 pp 835—851

Figure 3. Expression of novel miRNAs during DR. (A) The secondary structures of 3 predicted novel miRNA candidates as generated using the miRDeep2 program. (B) Expression of predicted novel miRNAs were examined in specific Argonaut gene mutants alg-1(gk214) or alg-2(ok304) by QRT-PCR (C) Expression changes of novel miRNAs in eat-2(ad1116) as compare to WT collected on day 1 of adulthood (young-adult worms) by qRT-PCR. Error bars indicate Standard Deviation between independent biological replicates. The p-values were calculated by t-test (* represents p < 0.05).