Research Paper Volume 6, Issue 8 pp 675—689

Figure 1. Daily rhythms in mTORC1in tissues of mice subjected to different feeding paradigms. Bars on the top of the figure represent the light (open bars) and the dark (black bars) parts of the day. ZT0 is the time when the light is turned on. (A, B and C) Representative Western blots (WB) of daily rhythms in phosphorylation of mTORC1 downstream targets: (A) in the liver of ad libidum (AL) fed mice; (B) in the liver of time restricted (TR) fed mice. The TR regimen is schematically depicted: 100% of the average daily ration was provided as a single meal at ZT14 (the white arrowhead); the food was consumed during three hours. (C) Quantification of phosphorylation of S6K1 and ribosomal S6 proteins on indicated sites normalized to total levels of the indicated proteins in the liver of AL (open diamonds, dashed black line) and TR (black triangles, solid black line) fed mice. 3 male mice per each time point and feeding regimen have been used. Data present Average +/− SEM; * p<0.05, statistically significant difference between different treatments.