Research Paper Volume 6, Issue 8 pp 645—660

Figure 1. The Gene-Switch GAL4 driver can be used to effectively knock down expression of Sin3A in adult Drosophila. RT-qPCR analysis was performed using tissue isolated from the indicated fly lines without RU486 (control) or with RU486 (RNAi knockdown). cDNA prepared from 25 day-old flies was used as a template in PCR with primer pairs for Sin3A. Numbers I and II refer to two distinct lines designed to express dsRNA to target different regions of the Sin3A transcript. Relative levels of gene expression are indicated. Error bars represent standard error of the mean. * p<0.01.