Research Paper Volume 6, Issue 8 pp 621—644

Figure 5. SKN-1 and DAF-16 are required for βHB-mediated lifespan extension. (A) βHB addition did not increase the lifespan of N2 worms fed RNAi to knockdown expression of skn-1. (B) βHB addition increased fluorescence of the gst-4::gfp SKN-1 reporter strain. Data are represented as mean +/− SEM (* p < 0.05). (C) βHB addition did not increase lifespan in daf-16(mgDf50) mutant worms. (D) βHB or butyrate increased fluorescence when administered to the sod-3::gfp DAF-16 reporter strain. Data are represented as mean +/− SEM (* p < 0.05).