Research Paper Volume 6, Issue 7 pp 575—586

Figure 2. Preadipocyte differentiation is preserved in long-lived GHR-/- mice. (A) Representative image of ING preadipocyte differentiation from 20-month old female wild-type mice following exposure to differentiation media for 48 hours. (B) Representative image of ING preadipocyte differentiation from 20-month old female GHR-/- mice following exposure to differentiation media for 48 hours. (C) PPARγ mRNA expression in differentiating ING preadipocytes from female GHR-/- and age-matched wild-type (WT) littermates. (D) C/EBPα mRNA expression in differentiating ING preadipocytes from female GHR-/- and age-matched wild-type (WT) littermates. E. aP2 mRNA expression in differentiating ING preadipocytes from female GHR-/- and age-matched wild-type (WT) littermates. Data were analyzed by Student's t-test and are expressed as mean ± SEM of 4 mice per group. Scale Bar: A-B=100μm; Arrows indicate differentiating cells, which contain doubly bi-refractile lipid droplets.^*P<0.05.