Research Paper Volume 6, Issue 6 pp 481—495

Figure 1. The dynamics of H₂O₂ penetration into hMESCs. (A) Intracellular ROS levels upon application of 200 µM H₂O₂. Cells were loaded with DCF, each trace represents the response of one cell. Three (N = 3) experiments were performed. (B) Fluorescent images taken at different stages (indicated by dashed lines) of the experiment illustrated in (A). Scale bar is 200 µm and valid for all images. (C) Intracellular ROS levels measured by FACS after staining with the fluorescent probe DCF. Values are means ± SD of three independent experiments. **p<0.005, ***p<0.001 versus control.