Research Paper Volume 6, Issue 5 pp 399—413

Figure 5. Microarray gene expression analysis of Mrc1⁺GFP^hi and Mrc1⁺GFP^lo cTM subsets and validation. (A) Flow cytometry contour plot of cardiac myeloid (CD45⁺CD11b⁺) cells from Cx₃cr1^GFP/+ mouse hearts with cTMs isolated for microarray analysis indicated (red dotted boxes). (B) Scatter plot of Mrc1⁺GFP^hi and Mrc1⁺GFP^lo gene probes ≥2.0-fold enriched (p≤0.05). Number of genes up-regulated or down-regulated in Mrc1⁺GFP^lo cTMs relative to Mrc1⁺GFP^hi cTMs are highlighted red or blue. (C) Receptors and membrane proteins up- or down-regulated in Mrc1⁺GFP^lo cTMs relative to Mrc1⁺GFP^hi cTMs. (D) Secreted factors up- or down-regulated in Mrc1⁺GFP^lo cTMs relative to Mrc1⁺GFP^hi cTMs. (E) Flow-cytometry analysis of differentially expressed cell surface markers (n=5 per marker). MFI = mean fluorescence intensity. (F) qRT-PCR analysis of differentially expressed genes (n≥4 per gene). NS = non-significant; *p<0.05; **p<0.01