Research Paper Volume 6, Issue 5 pp 399—413

Figure 3. Age-dependent decline in Cx₃cr1⁺ cTM cell density and proportion at the epicardium. (A) 45 μm confocal micrograph maximum intensity view of GFP and Mrc1 staining in mouse hearts sections from 4 and 30 week old Cx₃cr1^GFP/+ mouse heart sections (scale bar indicates 100 μm). a' and a'' are magnified views of areas demarked by dotted lines. (B) Histogram of proportion of GFP⁺ nuclei in relation to total nuclei in hearts of 4, 8 and 30 week old Cx₃cr1^GFP/+mouse heart sections. *p≤0.05. (C) Histogram of proportion of Mrc1⁺GFP^lo, Mrc1⁺GFP^hi or Mrc1⁻GFP^hi cTMs in relation to all cTMs at the epicardium. #p>0.05 Mrc1⁺GFP^hi vs Mrc1⁺GFP^lo; ¤p≤0.05 Mrc1⁻GFP^hi vs Mrc1⁺GFP^hi; ^p≤0.05 Mrc1⁻GFP^hi vs Mrc1⁺GFP^lo; *p≤0.05 Mrc1⁺GFP^hi vs Mrc1⁺GFP^lo; p>0.05 Mrc1⁻GFP^hi vs Mrc1⁺GFP^hi. All histograms show means ± SEM. Cell numbers were quantified from at least 4 fields of view from 3 mouse hearts/age group