Research Paper Volume 6, Issue 6 pp 496—510

Figure 2. Proliferative response of juvenile and adult utricular supporting cells to cyclin D1 overexpression. Explants were pulsed with EdU for 24 h between days 2 and 3, and analyzed at 3 and 7 DIV. (A,A') AdcD1-infected P6 utricle displays high numbers of EdU+/Sox2+ SCs. (B,B') AdcD1-infected P50 utricle shows lower numbers of EdU+ SCs. (C,C') Non-infected utricular explant is devoid of proliferating SCs. (D,D') The AdcD1-infected P6 utricle shows EdU+/cD1+ SCs (arrowheads). In addition, there are only EdU-positive and only cD1-positive SCs due to cell cycle dynamics and EdU pulsing (see Results). Abbreviations: AdcD1, adenovirus encoding cyclin D1; utr, utricle. Scale bar, shown in D': A-C', 20 µm; D,D', 8 µm.