Figure 1.Amino acids mediate lifespan and fecundity changes under DR(a) Summary of Drosophila median lifespans under dietary restriction (DR), full feeding (FF) and essential amino acid supplementation of DR (DR+EAA) (n=13 biological replicates; DR vs FF, P<0.001; FF vs DR+EAA, P=0.9383; DR vs DR+EAA, P=0.002; Wilcoxon rank-sum test) (b) A representative lifespan experiment: adding EAAs to DR food shortened lifespan (P< 0.001) to that of FF flies (P=0.194); n=150 per treatment; compared using the log-rank test. (c) Adding EAAs to DR food increased egg-laying (P<0.001) to that of FF flies (P<0.936). Fecundity: mean±s.e.m.; n=15; compared using the Wilcoxon rank-sum test
