Research Paper Volume 6, Issue 5 pp 369—379

Figure 3. Chronic L-arginine supplementation induces Arg-II expression/activity and mTORC1-S6K1 signaling. Cells were treated as described in Fig.1. (A) Western blot analysis of Arg-II expression and arginase activity assay. (B) Western blots showing activation of mTORC1 and S6K1 as monitored by phosphorylation of S6K1-T389 and S6-S235/236, respectively. The graphics at the corresponding right panels present the quantification of the signals. *p < 0.05 vs 0.1 mmol/L