Research Paper Volume 6, Issue 8 pp 602—620

Figure 6. (A) Quiescent satellite cells were isolated from uninjured muscle and plated into quiescent medium (DMEM with 1% horse serum final concentration [43]), with an equal volume of Opti-MEM conditioned by hESCs or Opti-MEM with the indicated FGFs at 30 ng/ml each, cultured for one day and then BrdU added overnight before fixation and immunoanalysis. Representative images are shown. Scale bar = 100 μM (B) Quantification. The activated satellite cells are the mean fraction of myogenic desmin +ve cells that are proliferating (BrdU+ve), normalized to the hESC condition, from three experiments, with − 1 SEM indicated (n=3). P-values are shown between FGF2 and FGF19, and FGF19 and FGF 2,6,19.