Increased mitochondrial biogenesis preserves intestinal stem cell homeostasis and contributes to longevity in <i>Indy</i> mutant flies

Ryan P. Rogers; Blanka Rogina

doi:doi:10.18632/aging.100658

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Research Paper Volume 6, Issue 4 pp 335—350

Increased mitochondrial biogenesis preserves intestinal stem cell homeostasis and contributes to longevity in Indy mutant flies

Figure 4. Indy mutations preserve intestinal integrity. (A) Electron micrograph of yw control (left) and Indy²⁰⁶/Indy²⁰⁶ (right) midgut imaged at 1000x. Arrow shows damaged tissue and asterisks mark ISCs. Scale bar represents 1 μm. (B) Quantitative analysis of blue staining of female control and Indy²⁰⁶/Indy²⁰⁶ flies fed food containing 2.5% w/v FD&C blue dye for 150 minutes at 40 days. Blue coloring throughout body indicates loss of intestinal integrity. Control and Indy²⁰⁶/dPGC-1^KG08646 flies have a significantly higher number of blue flies compared to Indy²⁰⁶/+ and Indy²⁰⁶/Indy²⁰⁶ mutants (p<0.01, n>50).