Research Paper Volume 6, Issue 6 pp 440—453

Age-related increase of VGF-expression in T lymphocytes

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Figure 4. Rapamycin-treated T cells down-regulate VGF. Isolated PBMCs were cultured in medium in the presence or absence of either Rapamycin or Cyclosporine A for 24h. Afterwards, the frequency of VGF-expressing CD3+ T cells was determined using flow cytometry (*p<0.05; one-way ANOVA).