Research Paper Volume 6, Issue 4 pp 281—295
Increased hepatic CD36 expression with age is associated with enhanced susceptibility to nonalcoholic fatty liver disease
Figure 5. Increased plasma membrane abundance of CD36 in aged mice fed a HFD is not related to Parkin expression levels. (A) Representative blot and (B) Immunoblot analysis to isolate plasma membrane (M) from cytoplasm (Cyt) using anti-CD36 antibody was performed in liver lysates from young and middle-aged mice fed a LFD and HFD. Anti-pan-Cadherin antibody was performed as a control for isolation purity and Actin antibody was performed as a control for protein loading. (C) Representative blot and immunoblot analysis using anti-Parkin antibody was performed in liver lysates from middle-aged mice fed a LFD and HFD and (D) in young and middle-aged mice fed a HFD. Tubulin antibody was performed as a control for protein loading. Values are expressed as mean ± SEM; * * p ≤0.05. (nonparametric Mann-Whitney U test).