Research Paper Volume 6, Issue 4 pp 281—295
Increased hepatic CD36 expression with age is associated with enhanced susceptibility to nonalcoholic fatty liver disease
Figure 4. Increased CD36 expression in aged mice fed a HFD for 12 weeks. (A) qRT-PCR measurement of Cd36 transcript levels in livers of young and middle-aged mice fed a HFD (versus young mice on LFD) and expressed as fold induction. (B) Immunoblot analysis using anti-CD36 and anti-tubulin antibodies was performed on liver extracts from young and middle-aged mice fed a HFD. (C) Immunoblots were quantified by densitometry and normalized against tubulin as a control for protein loading. Values are expressed as mean ± SEM; n = 6-8 mice in each group. * p ≤0.05. (nonparametric Mann-Whitney U test).