Research Paper Volume 6, Issue 4 pp 248—263
Methylated TRF2 associates with the nuclear matrix and serves as a potential biomarker for cellular senescence
Figure 5. The altered nuclear staining of methylated TRF2 is associated with altered nuclear structure in senescent cells. (A) Analysis of indirect immunofluorescence with anti-Lamin A/C antibody on young (p26) and senescent GM9503 (p48) cells. Cell nuclei were stained with DAPI in blue. (B) Quantification of percentage of GM9503 cells showing nuclear Lamin A staining that are either line-shaped or not associated with lines. A total number of 1014, 1050 and 1073 cells were scored for passages 21, 26 and 48, respectively. (C) Analysis of dual indirect immunofluorescence in late passage GM9503 (p45) with anti-TRF2-2meR17 antibody in conjunction with anti-Lamin A antibody. Cell nuclei were stained with DAPI in blue. (D) Analysis of dual indirect immunofluorescence in senescent GM9503 (p48) with anti-TRF2-2meR17 antibody in conjunction with anti-Lamin A/C antibody. Cell nuclei were stained with DAPI in blue. (E) Analysis of dual indirect immunofluorescence in senescent IMR90 (p40) with anti-TRF2-2meR17 antibody in conjunction with anti-Lamin A/C antibody. Cell nuclei were stained with DAPI in blue.