Research Paper Volume 6, Issue 4 pp 248—263
Methylated TRF2 associates with the nuclear matrix and serves as a potential biomarker for cellular senescence
Figure 4. The altered nuclear staining of methylated TRF2 is a general characteristics of replicative senescence. (A) Growth curve of IMR90 cells. IMR90 cells (p22) were passaged every four days continously for 72 days. (B) Quantification of percentage of IMR90 cells with BrdU incorporation. A total number of 952, 1027, 1146, 1720 and 1418 cells were scored for passages 27, 31, 35, 36 and 40, respectively. (C) Quantification of percentage of IMR90 cells with senescence-associated β-galactosidase staining. A total number of 1887, 1606, 1286, 1712 and 1047 cells were scored for passages 27, 31, 35, 36 and 40, respectively. (D) Analysis of indirect immunofluorescence with anti-2meR17 antibody in senescent IMR90 (p40) cells. Cell nuclei were stained with DAPI in blue. (E) Quantification of percentage of IMR90 cells with altered nuclear staining of methylated TRF2. A total number of 952, 1027, 1146, 1720 and 1418 cells were scored for passages 27, 31, 35, 36 and 40, respectively.