Research Paper Volume 6, Issue 4 pp 248—263
Methylated TRF2 associates with the nuclear matrix and serves as a potential biomarker for cellular senescence
Figure 2. Methylated TRF2 exhibits nuclear staining largely free of human telomeres. (A) Analysis of indirect immunofluorescence of three different cell lines with anti-TRF2-2meR17 antibody. Cell nuclei were stained with DAPI in blue. (B) Analysis of dual indirect immunofluorescence with anti-TRF2-2meR17 (green) in conjunction with anti-TRF1 antibody (red). HeLaI.2.11 cell nuclei were stained in blue. (C) Analysis of indirect immunofluorescence with anti-TRF2-2meR17 in conjunction with 100 ng of TRF2 peptide containing either modified or unmodified arginine 17. HeLaI.2.11 cell nuclei were stained with DAPI in blue.