Research Paper Volume 6, Issue 3 pp 231—245

Oxidative stress and antioxidant response in fibroblasts from Werner and Atypical Werner Syndromes

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Figure 4. Analysis of the peroxide detoxifying enzymes in atypical Werner syndrome and Werner syndrome fibroblasts. (A) Mean (±SD) mRNA levels of the CAT gene determined by qRT-PCR by triplicate. (B) Mean (±SD) mRNA levels of the GPX1 gene determined by qRT-PCR by triplicate. (C) Mean (±SD) mRNA levels of the GPX4 gene determined by qRT-PCR by triplicate. (D) CuZnSOD and MnSOD protein levels measured by Western blot in AWS, WS, and control fibroblasts.