Figure 4. Analysis of the peroxide detoxifying enzymes in atypical Werner syndrome and Werner syndrome fibroblasts. (A) Mean (±SD) mRNA levels of the CAT gene determined by qRT-PCR by triplicate. (B) Mean (±SD) mRNA levels of the GPX1 gene determined by qRT-PCR by triplicate. (C) Mean (±SD) mRNA levels of the GPX4 gene determined by qRT-PCR by triplicate. (D) CuZnSOD and MnSOD protein levels measured by Western blot in AWS, WS, and control fibroblasts.