Research Paper Volume 6, Issue 3 pp 207—214
Hexosamine pathway but not interstitial changes mediates glucotoxicity in pancreatic β-cells as assessed by cytosolic Ca2+ response to glucose
Figure 6. Effects of administration of azaserine and glucosamine in culture on subsequent [Ca2+]i responses to physiologic glucose in β-cells. A, Azaserine added in culture prevented the high (22.3 mM) glucose-induced impairment of [Ca2+]i responses to physiological glucose challenge. A representative of 36 cells. B, Glucosamine added in control culture with 5.6 mM glucose resulted in impairment of [Ca2+]i responses to physiological glucose challenge. N=15. C, Incidence of [Ca2+]i responses to 8.3 mM glucose in β-cells after culture for 3 days in described conditions. Numbers on top of bar indicate the number of cells that responded with [Ca2+]i increases over that examined.