Research Paper Volume 6, Issue 2 pp 131—139

Figure 2. Age-associated alterations in PMN surface markers. Whole blood of younger (n=32) and older (n =35) adults was stimulated with indicated ligands for 0, 15 min at 37 °C. Following RBC lysis, PMN were labeled for PMN markers at 4°C for 30 min. Flow cytometric labeling was detected by LSR II. Data shown are normalized MFI (Mean Fluorescence Intensity) of CD11b, CD18 and CD62L surface expression on PMN. Values indicate the means ± SEM in younger and older adults; asterisks indicate statistical significance between younger and older cohort (*P<0.05, **P < 0.01). For normalized CD11b and CD18 all stimulated PMN increased over time (P<0.001) and for CD62L all stimulated PMN decreased over time (P<0.001).