Research Paper Volume 6, Issue 2 pp 98—117

Figure 2. mdl-1 causes hyperplasia and hypertrophy. (A, B) mdl-1(0) causes oocyte stacking in 1 day old worms. (A) Nomarski images. (B) Quantitation of stacking. Sample sizes: N2, 23; mdl-1, 17. *** p < 0.001 (Student's t test). (C) No effect of mdl-1 on number of germline nuclei. p > 0.05 (Student's t test). (D, E) mdl-1 increases levels of germline apoptosis. (D) Epifluorescence images of SYTO12 stained cells in young adult hermaphrodite germline. (E) Quantitated data. Number of gonads scored: N2, 153; mdl-1, 132. *** p < 0.001 (Student's t test). (F, G)mdl-1 increases cytoplasmic streaming in the proximal gonad. (F) Single image obtained from a time-lapse recording. Arrows represent DIC-particle tracks. DIC-particles were tracked over a period of 1 minute. Scale bar: 20 μm. (G) Cytoplasmic streaming rate (mean particle speed ± standard error). 30 particle speed measurements performed for each genotype. Number of worms examined: N2, 4; mdl-1, 3. *** p < 0.001 (Student's t test). (H) Absence of effect of mdl-1(0) on gonad disintegration (25°C). p > 0.05 for all comparisons of N2 vs. mdl-1 of the same age (Wilcoxon Mann test). (I) Uterine status scale for quantitation of uterine tumor formation rate (5 classes). Class 1, normal uterus containing eggs (day 1 adult). Class 2, slightly abnormal uterine contents, but no tumor visible. Class 3, small tumor. Class 4, medium sized tumor. Class 5, large tumor, filling body cavity and squashing the intestine. Dotted line, outline of uterus. T, tumor. (J) mdl-1(0) increases uterine tumor formation (25°C), data summed from 3 trials. * 0.01 < p < 0.05 (Wilcoxon Mann test).