Research Paper Volume 6, Issue 1 pp 48—57

Figure 3. Increased mTORC1 signaling in tissues of Bmal1^−/− mice. Activity of TORC1 was compared in the different tissues of wild type and Bmal1^−/− mice. Protein phosphorylation in tissue extracts were analyzed by western blotting procedure with antibodies recognizing the indicated proteins or protein modifications or by in situ staining on 10μM frozen tissue sections. (a) In situ staining of cerebellum for p-S6 S235/236. (b) Phosphorylation of S6K1(T389) in the liver through the circadian cycle (wild type (black diamonds) and Bmal1^−/− (grey squares)). (c) and (d) Representative WB of mTORC1 downstream targets phosphorylation in the frontal cortex (c) and in the heart (d). Bars on the top of the figure represent light (open bars) and dark (black bars) parts of the day. Zt is an abbreviation for Zeitgeber Time, zt0 is the time when light is on, the time when light is off is zt12, thus, for example zt2 represents 2hrs after light is on. * - statistically significant difference between the genotypes