Research Paper Volume 5, Issue 12 pp 913—924

Figure 8. Human coronary artery EC display the anti-inflammatory phenotype. (A) HCAEC were either untreated (i) or treated with 0.2mM H₂O₂ (ii)and after 4 days fixed and stained for SA-β-gal. This is a representative of 3 separate experiments. Bar=100μm. (B) HCAEC were treated with 0.2mM H₂O₂ and after 4 days stimulated with 5ng/ml of TNFα for 5 hours, fixed and stained for p21 (green), E-selectin (red) and co-stained for DAPI (blue). Representative pro-inflammatory (i) and anti-inflammatory senescent cells (ii) are shown, highlighted by the white outline. This is a representative of 3 separate experiments. Bar=100μm.