Research Paper Volume 5, Issue 12 pp 913—924

Figure 6. The anti-inflammatory phenotype is stable and long lasting. (A) HUVECs were treated with 0.2mM H₂O₂ and after 8 or 16 days fixed and stained for SA-β-gal. This is a representative of 3 HUVEC lines. Bar=100μm. (B) HUVECs were treated with 0.2mM H₂O₂ and after 8 and 16 days fixed and stained for DAPI (blue) and p21 (green). This is a representative of 3 HUVEC lines. Bar=100μm. The percentage of induced pro and anti-inflammatory senescent cells on day 8 (C) and 16 (D) based on E-selectin and VCAM-1 expression were determined. This is a representative of the mean percentage +/− SD of 80 senescent cells from 3 HUVEC lines.