Research Paper Volume 5, Issue 12 pp 884—901

Figure 4. Anti-oxidant treatment restores proliferation and alleviates DNA damage in eIF2αA/A cells. (A) Primary eIF2αS/S and eIF2αA/A MEFs were maintained in the absence or presence of 200 μM Trolox (T). Cell proliferation for the indicated passages was assessed by cell counting. Data represent an average taken from two independent experiments performed in triplicates. (B) Primary eIF2αS/S and eIF2αA/A MEFs MEFs in the absence or presence of 200 μM Trolox in passage 3 were subjected to γ-H2AX staining and fluorescence microscopy. Nuclei were visualized by DAPI staining. Histogram represents the average number of γ-H2AX foci per cell (n=100).