Research Paper Volume 5, Issue 11 pp 835—849

Figure 4. SIRT4 regulates ATP in an ANT2-dependent manner. (A) ATP levels in HEK293T cells that were transfected with control, ANT2-siRNA, Sirt4-FLAG and Sirt4-FLAG/ANT2-siRNA constructs, as indicated. # indicates p < 0.05 for cells transfected with Sirt4-FLAG in the presence or absence of ANT2 (+/− ANT2-siRNA). (B) ATP levels in HEK293T cells that were transfected with control, ANT1-siRNA, Sirt4-FLAG and Sirt4-FLAG/ANT1-siRNA constructs, as indicated. Statistical significance was calculated using student's t-test and ANOVA: * p < 0.05, ** p < 0.01, *** p < 0.001 or as indicated. Error bars indicate mean values ± SEM.