Research Paper Volume 5, Issue 11 pp 835—849

Figure 2. Cellular respiration is negatively associated with Sirt4 expression. (A) Western blot showing liver Sirt4 expression during fed, fasted and refed conditions. (B) and (C) Oxygen flux (respiration) in control, Sirt4KD (B) and Sirt4OE (C) HEK293T cells. (D) Oxygen flux (respiration) in control and Sirt4KD HEK293T cells in media supplemented with 2mM pyruvate. (E) Oxygen consumption rate (OCR, respiration) in primary hepatocytes isolated from wild-type and Sirt4KO mice. OCR was measured under basal and oligomycin, FCCP and Rotenone/Antimycin-A treated conditions (media supplemented with 2mM pyruvate). (F) ECAR (glycolysis flux) in primary hepatocytes isolated from wild-type and Sirt4KO mice. Oxygen flux was measured under basal and FCCP treated conditions. Statistical significance was calculated using student's t-test: * p < 0.05, ** p < 0.01, *** p < 0.001 or as indicated. Error bars indicate mean values ± SEM.